| Molecular Plant Virology |
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| Group Leader |
Dr. S. K. Raj |
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Contact |
Work
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91-522- 2205831-35
Ext. 321 |
| Home |
91-522- 2733449 |
| Fax |
91-522-2205836 |
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Group works on
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Biological, biochemical and molecular
characterization of plant viruses affecting ornamental,
horticultural and crop plant.
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Development of diagnostics for plant viruses for their sensitive
detection in economically important crops.
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Development of transgenic plants for resistance against viruses.
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Objectives
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Authentic identification of virus isolates
for placing them in a proper group/ subgroup.
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Development of sensitive diagnostic kits for virus indexing of Indian isolates of Cucumoviruses and Geminiviruses in commercial crops.
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Genetic transformation of tobacco, chilli and tomato for raising of virus
resistant transgenic plants utilizing CP and Replicase gene of
Tomato Leaf Curl Virus and Cucumber mosaic virus.
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Achievements
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National
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- Plant viruses viz. Eggplant mottled crinkle virus (EMCV) from
Solanim melongena & S. capsicastrum, Chrysanthemum
aspermy virus (CAV) from Chrysanthemum and Cucumber mosaic virus
(CMV) from Chrysanthemum, Carnation, Amaranth, Datura, Gladiolus,
Banana, Tomato and Henbane have been characterized and their antisera
have been raised.
- Immuno-detection techniques viz. ELISA, ISEM, Western blot
assay have been standardized for detection of EMCV, CMV and
CAV. PCR for Tomato leaf curl virus in tomato, cucurbits, croton
& weeds and RT-PCR for Cucumber mosaic virus in Amaranth,
Chrysanthemum, Gladiolus, Banana, Tomato & Datura have been
developed for sensitive diagnosis.
- Coat protein and movement protein Cucumber mosaic virus (Amaranth)
and DNA-A of Pumpkin yellow mosaic virus were cloned and sequenced
for molecular characterization.
- Transformation of tomato using coat protein gene of CMV and
Tomato leaf curl virus has been done and transgenic plants expressing
coat protein of CMV and TLCV have been successfully generated.
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International
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- An isolate of Eggplant Mottled Crinkle Tombusvirus from Solanum
melongena and its polyclonal antibody deposited to the American Type
Culture Collections, USA.
- Sequence data of coat protein gene of CMV-Amaranth (AF 198622),
CMV-Datura (AF 281864), CMV-Henbane (AF 350450), movement protein of CMV-A
(AF 414078), coat protein region of TLCV-Pumpkin (AF 381977, AY 396151),
Acalypha yellow mosaic virus (AY 188956), Indian Croton gemini virus (AF
188965) and Tomato yellow leaf curl virus (AF 375241) submitted to GenBank.
- Collaboration with Scottish Crop Research Institute, Dundee, UK for
research on viruses associated with Groundnut Rosette disease of
groundnut.
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Research
Publications: 75
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National journals
International
Reviews in Book
Proceedings
TV talk
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: 35
: 30
: 03
: 05
: 02
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Feedback required
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- Groups working in this area in India as well as in abroad are
welcome for academic exchange.
- Industrial needs for commercialization of virus diagnostic kits
developed by the group.
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