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Microbiology
 
 
Group Leader Dr. Chandra Shekhar Nautiyal, Sci 'F'
Contact Work 91-522- 2206651
  Mobile 09415511111
  Email csnnbri@yahoo.com nautiyalnbri@lycos.com
 
 
Other Scientist of the Group

Dr. Suchi Srivastava, Sci ‘B’
 
 
Group works on

Working out the complexity of interaction between microbes and plants is one of the most complex scientific challenges. Under the present scenario, microbes will probably assume an increasingly prominent role as we look forward to new responsible technologies, if biotechnology is to evolve and provide inputs for both economic and environmental sustainability. The work being conducted is aimed at enhancing yield of economic crops by using native plant growth promoting rhizobacteria and developing stress tolerant transgenic bacteria and plants. Colonization of the plant root system is very important in nearly all interactions between plants and soil borne microbes. Selection and improvement of rhizosphere competent bacteria to encourage preferential colonization of desired bacteria can, therefore, be expected to modify plant health. Techniques of genetic engineering have been applied to encourage preferential colonization of the desired bacteria by engineering the plant environment specifically the rhizosphere to confer a superior ability to utilize a novel substrate, occurring in the “root” environment Noteworthy contributions are in the area of microbial biotechnology and relate to elucidation of relationships between microbial populations and environmental stresses, working out the intricacies of relationship between microbes and plants and utilizing the knowledgebase thus developed for enhancing the yield of economic crops through transfer of commercially exploitable technologies.
 

 
Objectives

  1. Development of efficient assays to evaluate the root colonization capability of the native rhizospheric bacterial population.
     
  2. Identification of plant beneficial native stress-tolerant nitrogen fixing and phosphate solubilising bacteria.
     
  3. Development of stress tolerant transgenic bacteria & plants, to enhance plant growth in degraded ecosystems.
     
  4. Identification and characterization of novel drought tolerant gene(s) from plant associated bacteria through expression studies, for engineering transgenic plants and microbes.
     
  5. Exploration and exploitation of microbial wealth of India for biotechnological prospects.
 
Achievements

  1. A simple sand-live soil assay method has been developed for large scale screening of the rhizosphere-competent bacteria, effective in suppressing plant pathogens. This invention demonstrates an increase in the efficiency of screening and detection of plant beneficial strains from the rhizosphere of plants.
     
  2. A method has been devised using which it is possible to screen quickly for superior rhizobia, which occur in nature. Correlation has been established between strain performance under abiotic environmental stress in pure culture and strain behavior under symbiotic condition. Gene(s) from stress tolerant Rhizobium have been cloned and are being used for creating stress tolerant transgenic bacteria and plants.
     
  3. A novel microbiological growth medium, National Botanical Research Institute's phosphate growth medium (NBRIP) has been defined, using which we have demonstrated that soil microbes should be screened in NBRIP broth assay for the identification of the most efficient phosphate solubilizers. Using this method, we have isolated phosphate solubilizing bacteria which efficiently solubilizers phosphate under extreme conditions of a biotic environmental stress.
     
  4. Molecular studies of stress sensing and response by using prometer less reporter gene insertions have been initiated to have a good understanding of the basis for host-strain interaction under stress. The work may be helpful in minimizing the impact of high salt, pH, and temperature stresses, currently limiting crop production under low-input conditions, and giving rise to more sustainable agriculture.

     
  5. Soil microbes represent an important source of bioactive compounds used in human therapy and agriculture. Exploration of microbial diversity for gainful purposes is being undertaken using function-driven analysis of metagenomic libraries to study the genome of 'unculturable' bacteria. It is anticipated that the work will allow us glimpse of the complexity of microbial communities and the huge, largely untapped, biotechnological resource that they represent.
 
Technology Transfers
 
  1. The "Antimicrobial NBRI1330 technology" based on the isolation and process, as described in our United States patent 6495362 has been licensed to a U.S. Biotechnology company.
     
  2. Bioinoculent technology has been transferred to Uttar Pradesh Government for its commercial production by Department of Agriculture's 17 biofertilizer and 9 biopesticide  manufacturing units.
     
  3. Bioinoculant technology has been transferred to Technology Information, Forecasting and Assessment Council (TIFAC), New Delhi for its large scale dissemination.
     
  4. Bioinoculant technology comprising novel microbial synergistic mixture of Bacillus sp. has been transferred for commercial production to Biotech International Ltd., New Delhi.
     
  5. Bioinoculant technology comprising novel microbial synergistic mixture of Bacillus sp. has been transferred for commercial production to Balaji Crop Care Pvt. Ltd., Hyderabad.
     
  6. Bioinoculant technology comprising novel of Pseudomonas putida has been transferred for commercial production to Balaji Crop Care Pvt. Ltd., Hyderabad.
 
National Awards

  1. Awarded for achievement in promotion of biotechnology All India Biotech Association (AIBA) Award for 2000-2001 by Hon'ble Minister of Science and Technology, Government of India.
     
  2. Awarded for significant contributions in Science, Vigyan Bharti Rashtriya Award for 2001 by Hon'ble Minister of Science and Technolgy, Government of India.
     
  3. Awarded the Biotech Product and Process Development and Commercialization Award 2004 by Hon'ble Minister of Science and Technology, Government of India.
 
Fellowships
 
  • Fellow of The National Academy of Sciences.
  • Fellow of National Academy of Agricultural Sciences.
 
Publications
 

Research Papers : 32
Review articles : 8
Books Edited : Editing 2 books for Springer, Germany on “Plant microbial co-existence” and “Microbes from extreme environments”
Patents : 18