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| Sustainable agro-forestry for Amelioration
of degraded soil sites & biomass productivity |
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Introduction
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Forestry has been a way of life in India
ever since the evolution of human beings through the forests. Uses
of trees for different purposes for their daily living including
wood, shelter and medicine have been the common feature. The fatigue
of green revolution and resource degradation leading to non-sustainable
production systems has demanded our attention for sustainable practices
to assure the continued production. In order to achieve this, scientific
systems of agroforestry were advocated and emphasized during 70s
and an initiative was made to sentisize the planners and scientists
to these aspects of tree based farming system. During the past 25
years there have been continuous attempts to inventorize, experiment
and synthesize scientific information on forestry/ agroforestry.
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Background
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| Eco-restoration was a focus area for
Prof. Kaul, the first Director of the Institute. His vision to create
field stations at Banthra set the path for eco-restoration of sodic
and degraded soil sites, one of the major problems in the state
of Uttar Pradesh. The country also faced a huge scarcity of wood
fuel. There was an urgent need to develop protocols for utilization
of degraded soil sites for wood-fuel production. Prof. T. N. Khoshoo,
other visionary Director emphasized on wood-fuel production on sustainable
basis. While research on alternative species for alkaline soils
was underway at Banthra Research Station, Professor Khoshoo pioneered
an experiment at a 35-acre at Banthra village (located about 20
km from Lucknow on Lucknow-Kanpur road) plot lying abandoned till
1981. This plot, known as ‘Football Ground’ with harsh,
unproductive and hostile soil, was converted to a Biomass Research
Center (BRC) that is a man made forest with research outputs oozing
from long term sustained experiments.
Financial support for the center came from the Ministry of Non
Conventional Energy Sources (initially known as Department of
Non-Conventional Energy Sources) and Council of Scientific &
Industrial Research, New Delhi. The program ran in phases initially
under the dynamic leadership of Late Dr. S.D. Khanduja (1981 -1986),
followed by Late Dr. P.D. Dogra, who was an authority on gymnosperms
particularly the blue pine (1986 -1988) and Dr. H.M. Behl (1988
- continuing). Dr. Behl was supported by Dr. (Mrs.) V.L. Goel
(Tree improvement) who is involved with this program since 1984;
Dr. B. Singh (Eco-restoration) and Dr. O.P. Sidhu (biofertilizers
& hydrocarbon crops), Dr. P.V. Sane and his group of Tree
physiologists, Dr. V.K. Garg and Dr. R.K. Jain (Soil Science),
Dr. C.S. Nautiyal, Dr. (Late) S. Surange (microbiology) and many
other scientists who contributed significantly to this program.
Currently the group includes Dr. Nandita Singh (Eco-auditing),
Dr. K. Chandrasekhar (Entomology), Sri. S.S. Tripathi (Field Management)
apart from Dr. V.L. Goel & Dr. O.P. Sidhu. In addition to
identification of suitable species and developing packages of
practices for cultivation of wood fuel tree & shrub species,
in short rotations, emphasis was given on basic and applied research
on seed source selection, root soil interaction, adaptation mechanism
developed by trees to cope with hostile surroundings, microbial
(rhizobial and mycorrhizal) associations, and sustainability of
system in terms of nutrient dynamics and soil amelioration.
The group was further supported by World Bank through the Jai
Vigyan NATP Program of the country for Germplasm collection, conservation
and evaluation.
Team of scientists with multidisciplinary R & D approach
and expertise undertook extensive and intensive research for higher
wood fuel productivity on alkaline soils through judicial selection
of germplasm, establishing high-tech nurseries, standardizing
slivicultural and tree breeding practices, soil and water management
etc.
The studies were carried out with societal mission for wasteland
development, poverty elevation and environmental conservation.
The major targets of the program were as below:
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- The generate knowledge on production of
woody biomass on degraded soil sites, through organic cultivation
without amendment of gypsum or pyrite.
- Identification of fuel-efficient species
for domestic and industrial purpose.
- Nursery Technology for production of quality
planting stocks.
- Standardization of slivicultural and tree
improvement practices for wood fuel tree & shrub species.
- Management of plantations and fixing harvest
rotations schedules to upgrade productivity per unit area.
- Study of soil amelioration, nutrient budgeting
and cycling.
- Study of sites specific microbial biofertilizers
for enhancing productivity.
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R and D Programmes and major achievements:
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Production of woody biomass
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In India fuel wood scarcity associated with continued
depletion of forest cover is a matter of serious concern. Forest
cover in India is far less than the national target of 33 %9.
Loss of tree cover leads to desertification, loss of biodiversity
and ecological imbalance including socio-economic crises. As biomass
is undoubtedly a viable energy option for the country, there is
an urgent need to expand afforestation program particularly on such
wastelands that are otherwise unfit for traditional agriculture7.
Need for such wood fuel plantation has further increased with possibilities
of conversion of biomass to energy via gasification and other conversion
forms1-3. Wood fuel marketing has remained neglected
since there are no structured markets in India. Wood fuel collection
is left to women folk of the house who collect wood for cooking
even at the cost of loss of mandays. However, with demand from industry,
woodfuel production may become an attractive business4, 8,
10.
Extensive work has been done at Biomass Research Centre of this
Institute for identifying promising species for fuel wood production
on substandard soil sites, improving their productivity through
silvicultural and tree improvement practices and ameliorating fertility
status of soil through nutrient cycling. However, in recent years
the concept of high-density plantation systems has gained immense
interest in order to efficiently combine and utilize the land, labour
and water resources for meeting immediate requirements of rural
communities for wood-fuel, fodder and timber including other minor
forest produce33.
Trials of several exotics and indigenous species (80)19-20
were laid during the past several years in moderate and high densities.
Species like Prosopis juliflora21, Acacia
nilotica, Leucaena leucocephala, Casuarina glauca
have shown good establishment and productivity (46 to 71 ha-1)
in 8 years rotation on these hostile soils. Other species such as
Terminalia arjuna, Acacia auriculiformis, Eucalyptus Dalbergia
sissoo, Pongamia pinnata, Albizia procera and Pithecellobium
dulce etc showed moderate performance in terms of adaptability
and yield potentials even in long rotations of 15 years (Table 1).
Harvest rotations including coppice rotations have been worked out.
Promising germplasm (seed sources and genotypes) suitable for afforestation
of sub-standard soils have also been identified. Species like Prosopis
tamarugo, Casuarina. obessa and Acacia demiaii could
not tolerate high sodicity of soils and completely failed to grow
on these soils. The residual effect of selected species on soil
properties was studied. The soil structure, physical and chemical
properties of the afforested soil changed notably in comparison
to the barren soils prior to plantation. A marked reduction in pH,
bulk density and sodium content; and manifold increase in the status
of carbon and calcium were observed indicating changes in fertility
status of these soils16. Extent of soil amelioration
varied greatly from species to species depending on the age, canopy
architecture, harvest rotations, litter fall, its decomposition
and mineralization36. Thus current studies suggest that
species selection and their silviculture is a very crucial task
in afforestation and bio-reclamation of sodic soils as many promising
species are available yet there are marked differences in their
productivity and potential to increase organic matter, soil nutrients
and under-story development (Figure 1).
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Figure 1 Wood fuel plantation at degraded sodic
soil site
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Microbial interventions particularly inoculation
of VAM fungi at nursery stage improved seedling growth and its potential
to survive and establish when planted in stress soils34-34.
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| Table 1: Performance of different species at 5-yr,
8-yr and 15 yr under trial at sodic soils |
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| Species |
Family |
Trial
age(Yr) |
Populationdensity
(stems ha-1) |
Stand
yield (t ha-1) |
Acacia auriculiformis
A. Cunn. Ex. Benth. |
Leguminosae
(Mimosoideae) |
15
8 |
2593
3650 |
130
54.54 |
Acacia nilotica Wild.
Ex. Del. |
Leguminosae
(Mimosoideae) |
15
8 |
619
1650 |
161
59.50 |
| Azadirachta indica |
Meliaceae |
5 |
4400 |
6.18 |
| Albizia lebbek Benth |
Leguminosae
(Mimosoideae) |
5 |
3175 |
0.46 |
Casuarina glauca Sieb.
Ex. Spreng. |
Casurinaceae |
8 |
3331 |
68.19 |
| Albizia procera Benth. |
Leguminosae
(Mimosoideae) |
7 |
2200 |
19.81 |
| Dalbergia sissoo Roxb. |
Leguminosae (Papilionoideae) |
8 |
1452 |
23.33 |
Eucalyptus hybrid |
Myrtaceae |
8 |
4950 |
20.91 |
Leucaena
diversifolia (Schlecht.) Benth. |
Leguminosae
(Mimosoideae) |
8 |
2124 |
24.12 |
Leucaena leucocephla
(lam.) de Wit* |
Leguminosae
(Mimosoideae) |
8 |
3999 |
70.1 |
Leucaena shannonii
Donn. Smith |
Leguminosae
(Mimosoideae) |
8 |
3506 |
39 |
| Pithecellobium dulce Roxb. |
Leguminosae
(Mimosoideae) |
15
5 |
2419
4200 |
66
1.29 |
| Pongamia pinnata Bennet. |
Leguminosae (Papilionoideae) |
8 |
954 |
6.49 |
| Prosopis juliflora Swartz. |
Leguminosae
(Mimosoideae) |
8
5 |
2875
4175 |
68.72
45.24 |
| Terminalia arjuna Bedd. |
Combretaceae |
5
8 |
4833
4380 |
3.51
19.45 |
| Sesbania formosa |
Leguminosae (Papilionoideae) |
5 |
2222 |
38.94 |
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An Indo-US Progam on Production of Woody Biomass
on sub-standard soil sites was successfully completed during 1984-1988.
High density short rotation trials of several species (Acacia
nilotica, A. cupressiformis and Syzigium cumini, Terminalia arjuna
etc.) were laid to develop protocols for production of biomass for
use in gasifiers for non-conventional energy.
The biomass production program was targeted for developing technologies
to produce biomass on wastelands which will provide energy for domestic
& industrial purposes24-30, provide employment by
wood-fuel marketing and provide environmental and energy securities
in rural India31. The program was made effective by organizing
information dissemination activities.
Highlights of the program were a State of Art Report on Wood Fuel
in 1998 (Dr. H.M Behl & Dr. P.V. Sane); organization of FAO
sponsored National meet of Forest officers and Director of Energy
Agencies of the Country (FAO proceedings published as a book in
2000); and National Seminar on Nursery Technology (1998). The Banthra
Research Model is a unique case study of sustainable biomass production
system on degraded lands.
The model developed at Biomass Research Center for sustainable utilization
of sodic soils is unique as it provides long-term effects of organic
cultivation of tree species. It does not utilize gypsum or pyrite
applications, it is completely organic, it involves tree improvement
as an integral component of plantation; and is model based on the
principals of soil amelioration and sustainability. It has added
substantially and economic value to the degraded soil.
The Biomass Research Center served the farmers and rural entrepreneurs
by providing technical support to end users by supplying quality
seeds and planting stocks (saplings and cloned materials) to public
and several government and non-government organizations; training
(National & International) on wood fuel production, management,
nursery technology, clonal propagation, land use systems, wasteland
afforestation, consultancy on availability of biomass and other
related aspects.
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Genetic selection and improvement of hard wood
tree species for fuelwood production on sodic soil with particular
reference to Prosopis juliflora
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Prosopis juliflora was found to be the most promising
tree species for wood fuel production on a variety of degraded soil
conditions5-6.
The species coppiced well and produced
shorter rotation with high productivity (Figure 2).
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Figure 2: Plantations of
Prosopis juliflora at high density,
multiple shoots, tree improvement
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A long term study on selection and improvement of
fast growing tree species suitable for wood fuel production on sodic
wastelands (pH 8.6–10.5) was undertaken. Field trials of nine
legumes (Acacia auriculiformis, A. nilotica, Albizia lebbeck,
A. procera, Dalbergia sissoo, Leucaena leucocephala, Pongamia pinnata,
Prosopis juliflora, Pithecellobium dulce) and three other tree
species (Azadirachta indica, Eucalyptus tereticornis and
Terminalia arjuna) were selected for this study. Prosopis
juliflora was the most promising species in terms of its biomass
productivity (68.7 tha-1) and fuel value index (148.8)
after 8-yr of growth13, 22. Acacia nilotica
ranked second. Intra-specific variations were screened at provenance
and individual tree level in order to improve fuelwood production
potential of P. juliflora through selection and breeding.
Successful populations (gene pools) and individuals (genotypes)
were cloned and conserved in clonal gardens to produce quality germplasm
for plantations on sodic wastelands. Genetic testing, selection
and multiplication of selected material are under progress. This
will optimise gains in future afforestation programmes on sodic
soils.
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Influence of planting density on growth and
biomass productivity of tree species for sodic soil sites
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Performance of three leguminous species (Acacia
farnesiana, A. nilotica subspecies cupressiformis
and Cassia siamea) was investigated at three planting densities
(10-, 20-and 30,000 plants ha-1) on a highly alkaline
soil site (pH 8.6 to 10.5) in order to identify promising species
and suitable plant spacing for optimum biomass harvest per unit
area under shorter rotation harvests (3 year). The study revealed
differential behaviour of various species in respect to plant growth,
survival and stand productivity in different population densities
(Figure 3).
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Figure 3: High density plantations
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Performance of A. farnesiana and C.
siamea in terms of plant height, stem diameter and plant establishment
was marginally affected by population density. Stand basal area
(2.4 to 6.4 m2 ha-1) and biomass (4.45 to 13.5 t ha-1)
in A. farnesiana increased markedly with increasing population
density. Similar gains in biomass were observed in Cassia siamea
when planted at higher densities. Individual tree biomass also was
not effected by increasing plant densities suggesting that these
two species respond well to high density plantation. A. nilotica
subspecies cupressiformis, on the other hand, showed a
negative response when planted in high density. Its biomass and
basal area decreased beyond 20,000 plants ha-1 planting
density suggesting that planting density of 20,000 plants ha-1
and above were supra optimal. Plants spaced at 10,000 plants ha-1
showed faster growth rate and higher productivity as compared to
same at 20- and 30,000 planting density Competition for space also
effected individual tree growth in higher densities. The concept
of high density plantation is not applicable in A. nilotica
subspecies cupressiformis. However, this species has significantly
greater potential since it has relatively high biomass production
even at low population density of 10,000 plants ha-1.
The study is useful in identifying productive species and optimum
plantation density per unit area for maximizing gains in terms of
biomass productivity in short rotation energy plantation programs
on sub-standard soil sites.
The productivity of Terminalia arjuna was investigated37-38
under varied plant spacing (10-, 20-, 30-, 40- and 50,000 trees
ha-1) on a highly alkaline soil site (pH 8.6 to 10.5)
in order to maximise biomass production and to assess optimum stocking
density. The species showed a very high survival percentage even
when planted in high densities. Closely spaced plants were significantly
taller than the more widely spaced plants of the same age. Mean
stand basal area and volume increased with increase in planting
density in the initial years but differences in basal area among
30- to 50,000 trees ha-1 treatments were not significant
at the age of three years. The trees at wider spacing had higher
individual biomass than those at closer spacing. The study showed
that after 4 years an optimum biomass of 22.7 t ha-1
was produced in the 30,000 trees ha-1 treatment. Densities
higher than 30,000 trees ha-1 were found to be supra-optimal
and resulted in over-crowding. The biomass produced in high density
plantation of T. arjuna was found suitable for use in gasifiers39
(Figure 4).
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Figure 4: High density
trails of Acacia farnesiana, Acacia nilotica cupressiformis
and Cassia siamea |
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Fuelwood production potential of Prosopis species
on an alkaline soil site
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The biomass potential of six species of Prosopis
was evaluated on highly alkaline soil site. Prosopis alba
I was found to have the fastest growth rate and highest above-ground
biomass production. P. juliflora ranked next. P. cineraria
showed high plant establishment but relatively slow growth. The
performance of P. glandulosa was poor on such sites. The
high fuelwood value index and rapid growth rate of P. juliflora
and P. alba makes them suitable for short-rotation fuelwood
forestry programmes on waste-lands. Selection of promising genotypes
is suggested as a means of improvement in yields.
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Fuelwood quality of promising tree species
for alkaline soil sites in relation to tree age
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The fuelwood quality of five tree species suitable
for afforestation of alkaline soil sites was investigated in relation
to tree age for establishing harvest rotation cycles12.
Prosopis juliflora and Acacia nilotica were found
to be the most suitable species for short rotation fuel wood forestry
programmes because of their high wood density, biomass yield, low
ash and moisture content, and good heat of combustion at the juvenile
stage23. The performance of other species like Acacia
auriculiformis, Terminalia arjuna and Sesbania formosa is discussed32.
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Case study of introduced Casuarina
species for biomass production
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Casuarina glauca is an introduced tree species
to India. It is one the few species that can grow normally in highly
sodic soil sites. Out of two exotic species of Casuarina (C.
glauca and C. obesa) investigated for biomass production
at highly sodic soil sites (pH 8.6 to 10.5); C. glauca
was found to be relatively promising in terms of growth and productivity.
Average height of plants in an 8-yr-old trial of Casuarina glauca
was 1033.3 ± 27 cm, however diameter at breast height (dbh)
remained at 8.59 ± 2.0 cm with a basal area of 6.68 ±
1.6 cm2 per plant. High plant survival and establishment
(75 %) indicate its potential for afforestation of degraded soil
sites. Linear regression equations (Y=a+bx) were developed to predict
biomass of standing stocks defining relationships between growth
parameters (x) as independent variable and productivity (Y) as dependent
variable. Both uni-factor equations based on one independent variable
(height or diameter alone), and multifactor involving both height
and diameter together (d2h) were derived to predict biomass of different
plant components.
Coefficient of correlation and regression coefficients
were found to be highly significant (p<0.001) in all the equations
irrespective of independent variable such as plant height, diameter
or both (d2h). There were marginal differences in R2
value (0.78 to 0.8) among equations derived by using d2
or d2h as independent variables. Height alone had relatively
poor functional correlation with yield (R2 = 0.45). Accordingly,
uni-factor linear equations with diameter (d2) were used
for computing stand biomass with reasonably good accuracy. At the
age of 8 years, stand productivity was 68.2 t ha-1 (oven
dry biomass) out of which relatively a very high proportion (80.3
%) of biomass was allocated to stem wood (54.8 t ha-1).
Both branch wood (8.4 t ha-1) and leaves (5 t ha-1)
contributed marginally. Casuarina glauca can be recommended
as a promising species for biomass production on alkaline soil sites
as is evident from its performance.
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Biofuel Mission program
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As part of the Coordinated program of the Department
of Biotechnology, agro-technologies of cultivating biofuel tree
species such as Pongamia pinnata, Madhuca indica,
so and Jatropha curcas (Figure 5). The program includes
study of variability, oil content and other traits for selection
of elite materials. Oil from the seeds of the tree species is extracted
for conversion to biodiesel. Biodiesel and bio oil for stationery
use has been developed under this program.
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Figure 5 Nursery of biofuel tree species
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Neem Network
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A National program on selection, cultivation and
improvement of neem was launched with the financial support of the
NOVOD Board18. NBRI is the National coordinator of the
program. Large germplasm was evaluated for various traits including
morphological.
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Nursery of neem
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A wide survey was made in different part of India
particularly in Uttar Pradesh14 and nine other states
for collection of seeds (>70 provenances). Striking variations
among populations and genotypes with respect to tree form (straight,
crooked and deformed) seed l size and morphology (oval, oblong,
rounded, elongated and ribbed) and chemical constituents particularly
oil content (19.8% to 53.5 %) and azadirachtin contents (301.8 mg/kg
to 3161.4 mg/kg kernel) were recoded. Several selected phenotypes
with unique traits were multiplied vegetatively and conserved in
clonal gardens as a future resource of germplasm for large scale
commercial plantations.
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Figure 6: Nursery of selected material of neem
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High Tech Nurseries
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The group has developed expertise in
High Tech (Low cost) Nurseries. This expertise is coupled with R&D
inputs to develop clonal propagation protocols particularly for
difficult to root species of horticulture, wood fuel, oil yielding
and ornamental species15. The technology has been up
scaled to commercial production and is provided as a package, turn-key
project or consultancy (Figure 7).
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Figure 7: High Tech nursery for clonal propagation
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Protocols were developed for large scale multiplication
and clonal propagation of difficult to root species of horticulture17,
wood fuel, biofuel tree species and other plants of economic importance.
The major breakthroughs are: |
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- Protocols for clonal propagation were developed for several
horticulture, forest and medicinal plant species.
- Protocols for hardening of tissue culture raised or macro-propagated
saplings.
- Turn key cum consultancy project for Forest Department, Hanumangarh
(Rajasthan) for clonal propagation of poplars and Dalbergia
sissoo.
- Turn key cum consultancy project for Forest Department, Bikaner
(Rajasthan) for clonal propagation of Prosopis cineraria &
Neem.
- Consultancy project with UPBSN (World Bank) Lucknow for forest
and horticulture species and developing entrepreneurship in
Uttar Pradesh.
- Turn key cum consultancy project for Forest Department, Jaisalmer
(Rajasthan) for clonal propagation of Tecomella undulata and
neem.
- Turn key cum consultancy project for Forest Corporation,
Uttar Pradesh for clonal propagation of teak and other forest
species.
- Turn key cum consultancy project doe FFDC, Kanpur for propagation
of medicinal plants.
- Collaborative program with Bio Technology Park, Lucknow for
hardening of tissue culture raised plants.
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Exploration, Collection and Evaluation of Agroforestry
tree species
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Biodiversity assessment and conservation is a national
priority. NBRI is one of the 11 active centers of NBPGR for accessioning
and conservation of biodiversity under Jai Vigyan NATP Program of
the country. The importance of this programme has increased with
the release of `Plant Varieties and Farmer’s Rights Act 2001’
which has set the stage to usher in an intellectual property rights
regime in agriculture.
In germplasm conservation, the Program is concerned with the conservation
of genetic diversity in plant species, populations, genotypes, which
are actually or potentially useful to mankind. It is not known that
actually or what all characters/types will be of use in the distant
future. But what best one can foresee is being conserved. NBRI program
includes accessioning of species suitable for agro-forestry, bio-fuels,
dye resources, medicinal and ornamental plants.
Thorough covering central, western and eastern U.P., Rajasthan,
Uttranchal , North Bihar, Jharkhand and M.P were undertaken to collect
for agroforestry tree species (e.g., Azadirachta indica, Acacia
nilotica, Sesbania sps., Albizia lebbeck, Albizia procera, Pongamia
pinnata, Salvadora etc.); medicinal plants (e.g., Deploknemia
butyraceae, Jatropha curcas, Nyctanthus arbostris, Withinnia somnifera,
Rubia cardifolia, Piper beetle, Aloe vera, Asparagus racemosus
etc.) and ornamentals (e.g., Tagetes erectus, Gillardia sps.,
Crysynthemum, Hibiscus rosachinensis, Tabelia sps., Plumelia alba).
The plants showed variability in their habitat, form and chemical
constitution.
The germplasm collected is accessioned and send to NBPGR for cryo
preservation. The vegetative propagated germplasm is being maintained
in the nursery at NBRI.
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Acknowledgements
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The group is grateful to the Ministry of Non Conventional
Energy Source, Govt. of India, Department of Science & Technology,
NATP (ICAR), Ministry of Agriculture, NOVOD Board and other organizations
who provided financial support for the programme.
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Literature cited
|
- Behl H.M. (1992). In Proc. IUFRO Centennial Meeting, Eberswalde,
Germany, August 31st to September 6th, 1992.
- Behl H.M. (1993). In: Managing Biodiversity for Energy (Ed.
P. Chaturvedi) World Food Day Publication, FAO and IAAS publication,
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- Behl H.M. (1995). In: Proc. National Seminar on Plantation
in Wastelands, Delhi, pp 49 - 50.
- Behl H.M. (1996). In: Proc. Int. Conference, 9th European
Bioenergy Conference, June 24-27 1996, Copenhagen, Denmark.
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- Behl H.M. and Goel, V.L. (1992). In: Proc. IUFRO ALL Division
5-Conference (Wood quality), Nancy, France, August 23-28 1992.
- Behl H.M. and Goel, V.L. . (1998). In: Tewari, J.C., N.M.
Pasiecznik, L. N. Harsh and P.J.C. Harris (eds.) 1998. Prosopis
species in the Arid and Semi-arid Zones of India. Proceedings
of a Conference, 21-23 November (1993), CAZRI, Jodhpur, India.
The Prosopis Society of India and the Henry Doubleday Research
Association, 55 – 62 p.
- Behl H.M. & Goel, V.L. (2000). In: Collection, Processing
and Commercial Utilization of Neem (Eds. A. Lahri, H.M. Behl),
Fragrance & Flavour Development Center, Govt. of India,
pp. 267 - 271.
- Behl H.M. & Goel, V.L. (2002) A technical manual on High
Tech Nursery for clonal propagation.
- Behl H.M., Goel, V.L. and Sidhu, O.P. (1992). In: Proc. International
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Food Sciences and Nutrition, 48:215 - 219.
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Agricultural Biochemistry Vol. 10: 87 - 22.
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& Bioenergy, 17 (3): 273 -278.
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Agricultural Biochemistry. 9 (1&2): 8 - 10.
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Biodiversity collections
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