| Planning
& Coordination |
| |
| |
| |
| |
Plant
Physiology
|
| |
Objectives
|
To
impart training to prospective students and faculty in methods
of biotechnological, physiological and biochemical research methods.
|
| |
Training Methodology
|
Following
modules are offered.
|
Module I Training in Tissue Culture Techniques
|
This module provides on hand training in tissue
culture techniques in plants, somatic embrygenesis and other
aspects of culturing plants and lower organisms like cyanobacteria.
|
| |
Module II Protein isolation, purification
and characterization
|
This course module provides training in different
methods of protein isolation, purification and characterization
especially of different enzymes of nitrogen assimilation (NR,NIR,
GS) and essential amino acid biosynthesis, anti-oxidative defense
related enzymes such as peroxidases, catalases, superoxide dismutase
glutathione reductase and stress related enzymes and proteins
including metal binding proteins. The techniques covered include
training in protein isolation estimation, enzyme activities,
column chromatographic techniques (gel filtration, ion exchange
and affinity), molecular mass determination of proteins by gel
permeation and SDS PAGE. Training in finer methods of protein
characterization and expression through immunological and proteomics
is also offered.
|
| |
Module III Methods in photosynthesis research
and plant productivity
|
This module provides training in methods of
photosynthesis measurements including PS I and PS II activities,
field photosynthesis and plant productivity using modern tools LICOR Photosynthesis Systems, Fluorescence oxygen electrodes.
|
| |
Module IV Methods of estimation of plant metabolites
|
This module provides training in the estimation
of plant pigments- chlorophylls, carotenoids and xanthophyll cycle,
anthocyanins, betalains , amino acids, simple and polyphenols
and sugars.
|
| |
Module V Agro-techniques including propagation
techniques in betel vine cultivation, Gladiolus cultivation and
chrysanthemums
|
This course module provides training in agrotechniques
in plant propagation and cultivation of betel vine (valuable for
its leaves), gladiolus corms and chrysanthemums. The techniques
covered also include culturing of plants in solution culture (hydroponics).
|
| |
Coverage of theoretical and practical aspects
|
All the modules given above will cover the theoretical
and practical aspects including on hand training.
|
| |
Anticipated level of the participants
|
Graduates in sciences and medicine, post- graduates,
doctoral students and post doctoral fellows.
|
| |
Facilities available in lab for training
|
-
Tissue culture labs with sate of the art facilities.
-
Systems for protein purification , columns, electrophoresis
equipments for native and SDS PAGE, UV- visible spectrophotometers,
FPLC, cold labs etc are available.
-
Equipments for photosynthesis research such as LICOR systems,
IRGA, Fluorescence electrodes are available.
-
Facilities for agro techniques, land, glass house, net house
for growing plants are available.
|
| |
Faculty/Expertise
|
Dr.
V. K. Kochhar
Area Coordinator
Biotechnology & Plant Physiology
National Botanical Research Institute
Rana Pratap Marg
Lucknow-226001 (UP), India |
|
| |
|
|
